Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">qPCR Control Plate for mCherry & GAPD targets</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">qPCR Control Plate for mCherry & GAPD targets</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Latest revision as of 00:55, 27 September 2017
qPCR Control Plate for mCherry & GAPD targets | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OverviewTo establish some quality control standards for the RNA-seq and qPCR experiments, I performed qPCR on all dilutions of all templates using mCherry & GAPD as targets. Templates to be used are:
Also will be trying out a duplex reaction with GAPD & mCherry primers in the same sample, using a template that shows strong mCherry signal. qPCR plate layout for U2OS & KAH126-U2OS templatesqPCR results for U2OS & KAH126-U2OS templatesExperiment: DualColorProbe_UPL_DNyer042215 Active filters: FAM (465-510), VIC / HEX / Yellow555 (533-580)
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