Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/15: Difference between revisions
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[[Image:2015-04-15_KAH187-MV8_digest_annotated.png]] | [[Image:2015-04-15_KAH187-MV8_digest_annotated.png]] | ||
Expected size fragments from EcoRI/ApaLI digestion: 3951, 1243, 1135, 288, 234. | |||
Gel was run a little too long, ~300bp fragment sizes are difficult to visualize. | |||
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Revision as of 13:31, 16 April 2015
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||
Plasmid Maxiprep of KAH187-MV8--Notes-- During the plasmid purification procedure, I accidentally mixed the lysate flowthrough (containing plasmid) with the column preparation solution that was run through the Binding Column. Proceeding as normal from this point forward. Hopefully doesn't influence yield. --DNA Quantification-- Measured using the Take3 plate:
--Restriction Enzyme Digest--
Digest for 15 minutes at 37°C. --Gel Electrophoresis-- Expected size fragments from EcoRI/ApaLI digestion: 3951, 1243, 1135, 288, 234. Gel was run a little too long, ~300bp fragment sizes are difficult to visualize.
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