Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/04/15: Difference between revisions
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<b>--Gel Electrophoresis--</b> | <b>--Gel Electrophoresis--</b> | ||
[[Image:2015-04-15_KAH187-MV8_digest_annotated.png | [[Image:2015-04-15_KAH187-MV8_digest_annotated.png]] | ||
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Revision as of 18:21, 15 April 2015
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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Plasmid Maxiprep of KAH187-MV8--Notes-- During the plasmid purification procedure, I accidentally mixed the lysate flowthrough (containing plasmid) with the column preparation solution that was run through the Binding Column. Proceeding as normal from this point forward. Hopefully doesn't influence yield. --DNA Quantification-- Measured using the Take3 plate:
--Restriction Enzyme Digest--
Digest for 15 minutes at 37°C. --Gel Electrophoresis--
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