Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/27: Difference between revisions
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* ✓ Measure cell density of K562 culture via flow cytometry | * ✓ Measure cell density of K562 culture via flow cytometry | ||
* If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | * ✓ If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid | ||
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Revision as of 15:16, 27 February 2015
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryIn paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.
Goals
Protocoltransfection with lipofectamine
NotesWell 1: KAH126/MV2, ben's plasmid prep (original concentration 377 ng/μL)
Results/ConclusionsCulture density is 668,000 cells/mL as measured via flow cytometry.
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