Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/27: Difference between revisions

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(Autocreate 2015/02/27 Entry for Haynes_Lab:Notebook/RNA-seq_of_PcTF_Transfected_U2OS_&_SK-N-SH_cell_lines)
 
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==Goals==
==Goals==
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* List goals here, in list format
* Measure cell density of K562 culture via flow cytometry
* If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid


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Revision as of 14:37, 27 February 2015

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.



Goals

  • Measure cell density of K562 culture via flow cytometry
  • If cell density is between 500,000 and 1,000,000 cells/mL, transfer to fresh antibiotic-free medium and transfect with lipofectamine, KAH126/MV2 plasmid



Protocol

example protocol




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Data collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.