Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/02/26: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Goals==
==Goals==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* List goals here, in list format
* &#x2713; Measure K562 cell density via FC
* &#x2713; Passage K562 cell line


<br><br>
<br><br>


==Protocol==
==Protocol==
[http://openwetware.org/wiki/Haynes:ThawingCells example protocol]
[http://openwetware.org/wiki/Haynes:SplittingCells passaging cells]
 
[http://openwetware.org/wiki/Haynes:FCMamalian_FluorGene flow cytometry]




<br><br>
<br><br>
==Notes==
==Notes==
Observations, measurements, placeholder info etc. go here.
Observations, measurements, placeholder info etc. go here.
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==Results/Conclusions==
==Results/Conclusions==
Data collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.
Cell density measured at 287,000 cells/mL. This is a 3-fold increase from yesterday's counts; at this rate, cells will be ready for transfection tomorrow.
 


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Latest revision as of 00:47, 27 September 2017

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines Main project page
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Summary

In paragraph format, state what you are going to do today so a reader unfamiliar with your project could understand what is going on.



Goals

  • ✓ Measure K562 cell density via FC
  • ✓ Passage K562 cell line



Protocol

passaging cells

flow cytometry




Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Cell density measured at 287,000 cells/mL. This is a 3-fold increase from yesterday's counts; at this rate, cells will be ready for transfection tomorrow.



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