Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/29

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Summary

Today I'll be performing another transfection of SKNSH samples, as well as purifying plasmid and running it on a gel to check for purity.



Goals

  • SKNSH transfection with lipofectamine
  • Plasmid mini-prep
  • Plasmid restriction digestion
  • Run digestion products on agarose gel to check for purity



Protocol

transfection with lipofectamine

Restriction digestion setup:

Reagent Volume
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1
PstI 1
dH2O 9.5
Total 15 μL

Incubate for 30min at 37°C, then run on a 1% agarose gel with 1kb ladder.



Notes

Observations, measurements, placeholder info etc. go here.



Results/Conclusions

Data collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.



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