Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/29: Difference between revisions
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==Protocol== | ==Protocol== | ||
[http://openwetware.org/wiki/Haynes: | [http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo transfection with lipofectamine] | ||
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Restriction digestion setup: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume''' | |||
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| DNA(plasmid)||2.0 μL | |||
|- | |||
| 10X buffer||1.5 | |||
|- | |||
| EcoRI||1 | |||
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| PstI||1 | |||
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| dH2O||9.5 | |||
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| Total||15 μL | |||
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Incubate for 30min at 37°C, then run on a 1% agarose gel with 1kb ladder. | |||
<br><br> | <br><br> | ||
==Notes== | ==Notes== | ||
Observations, measurements, placeholder info etc. go here. | Observations, measurements, placeholder info etc. go here. |
Revision as of 16:59, 29 January 2015
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||
SummaryToday I'll be performing another transfection of SKNSH samples, as well as purifying plasmid and running it on a gel to check for purity.
Goals
Protocoltransfection with lipofectamine Restriction digestion setup:
Incubate for 30min at 37°C, then run on a 1% agarose gel with 1kb ladder.
NotesObservations, measurements, placeholder info etc. go here.
Results/ConclusionsData collected from your experiments, significance of findings, future steps etc. all go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.
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