Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/22: Difference between revisions
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==Results/Conclusions== | ==Results/Conclusions== | ||
Fluorescence microscopy images of KAH126/U2OS culture induced on Monday are below. | |||
[[Image:2015-01-22_Well_1_phase_%26_mCherry_10x.jpg | 500px | Well 1]] | |||
[[Image:2015-01-22_Well_2_phase_%26_mCherry_10x.jpg | 500px | Well 2]] | |||
[[Image:2015-01-22_Well_3_phase_%26_mCherry_10x.jpg | 500px | Well 3]] | |||
[[Image:2015-01-22_Well_4_phase_%26_mCherry_10x.jpg | 500px | Well 4]] | |||
[[Image:2015-01-22_Well_5_negative_control_phase_%26_mCherry_10x.jpg | 500px | Well 5 (negative control)]] | |||
[[Image:2015-01-22_Well_6_negative_control_phase_%26_mCherry_10x.jpg | 500px | Well 6 (negative control)]] | |||
Revision as of 17:21, 22 January 2015
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryToday the U2OS culture in 6-well plates looks ready for transfection (~80% confluency) and the KAH126/U2OS culture in 6-well plates looks ready for induction by doxycycline (~80% confluency). I'll be taking care of that, as well as running a plasmid mini-prep on some E. coli cells I grew up and harvested earlier in the week.
Goals
Protocol
NotesObservations, measurements, placeholder info etc. go here.
Results/ConclusionsFluorescence microscopy images of KAH126/U2OS culture induced on Monday are below.
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