Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/22: Difference between revisions
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==Protocol== | ==Protocol== | ||
[http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo mammalian tranfection w/lipofectamine] | * [http://openwetware.org/wiki/Haynes:TransfectionPlasmid_Lipo mammalian tranfection w/lipofectamine] | ||
* Doxycycline induction of KAH126/U2OS cells - just add 1uL of doxycycline per mL of medium in each sample to be treated. | |||
Doxycycline induction of KAH126/U2OS cells - just add 1uL of doxycycline per mL of medium in each sample to be treated. | * Plasmid prep - follow instructions in the Sigma plasmid mini-prep and in the cloning bootcamp | ||
Plasmid prep - follow instructions in the Sigma plasmid mini-prep and in the cloning bootcamp | |||
Revision as of 17:13, 22 January 2015
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryToday the U2OS culture in 6-well plates looks ready for transfection (~80% confluency) and the KAH126/U2OS culture in 6-well plates looks ready for induction by doxycycline (~80% confluency). I'll be taking care of that, as well as running a plasmid mini-prep on some E. coli cells I grew up and harvested earlier in the week.
Goals
Protocol
* Plasmid prep - follow instructions in the Sigma plasmid mini-prep and in the cloning bootcamp
NotesObservations, measurements, placeholder info etc. go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.
Results/ConclusionsData collected from your experiments, significance of findings, future steps etc. all go here.
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