Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2015/01/22: Difference between revisions
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==Goals== | ==Goals== | ||
* | * Transfect U2OS cells | ||
* Induce KAH126/U2OS cells | |||
* purify plasmid from E. coli | |||
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Revision as of 17:11, 22 January 2015
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryToday the U2OS culture in 6-well plates looks ready for transfection (~80% confluency) and the KAH126/U2OS culture in 6-well plates looks ready for induction by doxycycline (~80% confluency). I'll be taking care of that, as well as running a plasmid mini-prep on some E. coli cells I grew up and harvested earlier in the week.
Goals
Protocol
NotesObservations, measurements, placeholder info etc. go here. Like the introduction of the page, this should also be in paragraph format and summarize your findings so a new reader can follow along.
Results/ConclusionsData collected from your experiments, significance of findings, future steps etc. all go here.
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