Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/24: Difference between revisions
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==Materials== | ==Materials== | ||
* | * 70% ethanol spray bottle | ||
* | * 1x Phosphate-buffered saline (PBS) | ||
* Trypsin-EDTA buffer/ media | |||
* Complete cell culture medium (e.g., 10% FBS, 1% pen-strep) | |||
* Sterile tissue culture-treated vent-capped T-75 flask, one per new culture | |||
* Pipette tips, etc. | |||
* 100% confluent (dense) cell culture (check confluency under the light microscope) | |||
==Notes== | ==Notes== |
Revision as of 13:18, 24 December 2014
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryThe second transfection attempt is also showing extremely low levels of PcTF-mCherry protein in the U2OS cell line (fluorescence microscopy images not shown here). Today being Christmas Eve, I'm going to wait until next week before making another attempt. Goals
ProtocolMaterials
NotesObservations, measurements, placeholder info etc. go here Results/ConclusionsAny significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
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