Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/23

From OpenWetWare
Jump to navigationJump to search
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

Today we'll be harvesting the U2OS cells from the first transfection attempt. Fluorescence microscopy of the cells indicates there is little or no PcTF-RFP expression in the line, but we'll be doing a run through with flow cytometry and RNA purification regardless, as practice for future attempts.

Goals

  • Harvest U2OS cells from 1st transfection attempt
  • Flow cytometry on cells to determine gene expression levels
  • RNA purification on cells to create cDNA library (see below, Results section)

Protocol

example protocol

Materials

  • List materials here, to be gathered before the experiment
  • Consumables such as media, antibiotics, DNA, kits etc.

Notes

Observations, measurements, placeholder info etc. go here

Results/Conclusions

Any significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/RNA-seq_of_PcTF_Transfected_U2OS_%26_SK-N-SH_cell_lines/2014/12/23&oldid=848703"