Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/23: Difference between revisions
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Overview of flow cytometry data:<br><br> | Overview of flow cytometry data:<br><br> | ||
[[Image:20141223_flow_cytometry_overview.png | thumb]]<br><br> | [[Image:20141223_flow_cytometry_overview.png | thumb]]<br><br> | ||
Negative control does not have significantly different fluorescence values than the samples (data not shown). | Negative control does not have significantly different fluorescence values than the samples (data not shown).<br><br> | ||
==Results/Conclusions== | ==Results/Conclusions== | ||
Revision as of 15:25, 23 December 2014
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryToday we'll be harvesting the U2OS cells from the first transfection attempt. Fluorescence microscopy of the cells indicates there is little or no PcTF-RFP expression in the line, but we'll be doing a run through with flow cytometry and RNA purification regardless, as practice for future attempts. Goals
Protocolflow cytometry of mammalian cells for measuring fluorescent gene expression Materials
NotesOverview of flow cytometry data:  Negative control does not have significantly different fluorescence values than the samples (data not shown). Results/ConclusionsAny significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
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