Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/23: Difference between revisions
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==Materials== | ==Materials== | ||
* | * PBS buffer | ||
* | * Trypsin / EDTA | ||
* Complete cell medium | |||
* FACS Buffer - 1% FBS in 1x PBS (stored at 4°C) | |||
* FACS tubes with strainer caps - 6 mL capacity (EMS 64750-25) | |||
* TRIzol / RNA purification miniprep & supplies | |||
* pipette tips, etc.<br><br> | |||
==Notes== | ==Notes== | ||
Revision as of 15:10, 23 December 2014
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryToday we'll be harvesting the U2OS cells from the first transfection attempt. Fluorescence microscopy of the cells indicates there is little or no PcTF-RFP expression in the line, but we'll be doing a run through with flow cytometry and RNA purification regardless, as practice for future attempts. Goals
Protocolflow cytometry of mammalian cells for measuring fluorescent gene expression Materials
NotesObservations, measurements, placeholder info etc. go here Results/ConclusionsAny significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
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