Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Summary== | ==Summary== | ||
Here are some pictures taken of the cells after the second transfection attempt. Efficiency is still low, but we'll be expanding the lines regardless.<br><br> | |||
==Goals== | ==Goals== | ||
* | * Expand U2OS transfected cell lines (from 2nd attempt) | ||
* Check SKNSH cell line, passage if necessary<br><br> | |||
==Protocol== | ==Protocol== | ||
[http://openwetware.org/wiki/Haynes: | [http://openwetware.org/wiki/Haynes:SplittingCells passaging cells]<br><br> | ||
==Materials== | ==Materials== | ||
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==Notes== | ==Notes== | ||
SKNSH cells to be passaged from PS-β to PS-1, 5-fold dilution.<br><br> | |||
==Results/Conclusions== | ==Results/Conclusions== | ||
Composite photographs (phase contrast & mCherry red fluorescence) of U2OS cultures transfected with PcTF-mCherry plasmid:<br><br> | |||
[[Image:20141222_Well_1_10x_phase_&_mCherry.jpg|900px|]]<br><br> | |||
[[Image:20141222_Well_2_10x_phase_&_mCherry.jpg | 900px|]]<br><br> | |||
[[Image:20141222_Well_3_10x_phase_&_mCherry.jpg | 900px|]]<br><br> | |||
[[Image:20141222_Well_4_neg_control_10x_phase_&_mCherry.jpg | 900px|]]<br><br> | |||
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__NOTOC__ | __NOTOC__ | ||
Latest revision as of 00:36, 27 September 2017
 RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines
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SummaryHere are some pictures taken of the cells after the second transfection attempt. Efficiency is still low, but we'll be expanding the lines regardless. Goals
ProtocolMaterials
NotesSKNSH cells to be passaged from PS-β to PS-1, 5-fold dilution. Results/ConclusionsComposite photographs (phase contrast & mCherry red fluorescence) of U2OS cultures transfected with PcTF-mCherry plasmid:
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