Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/18
RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
SummaryThe transfection from earlier in the week had a very low efficiency, but we're going to expand that cell line just in case, transferring the cells into 10cm petri dishes. Meanwhile, we'll also be setting up another batch of U2OS cells for transfection by advancing them to another 6 well plate, as well as passaging the line from PS-1 to PS-2. Lastly, we'll be using the PS-1 backup flask to create more frozen stock of U2OS. Goals
Protocolpassaging adherent cells - for passaging cells from PS-1 to PS-2
To advance the transfected U2OS cells from 6-well plates to 10cm petri dishes:
Materials
NotesObservations, measurements, placeholder info etc. go here Results/ConclusionsAny significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
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