Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/16

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RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

Explain what you're doing today and why. Paragraph format, simple description so somebody reading the notebook has some orientation.

Goals

  • Begin transfection of U2OS cells in 6-well plate

Protocol

mammalian cell transfection with liopfectamine

Materials

  • Lipofectamine LTX
  • Opti-MEM I Reduced serum medium
  • Antibiotic-free growth medium (no pen/strep or other antibiotics)
  • Cells
  • Pipette tips

Notes

Observations, measurements, placeholder info etc. go here

Results/Conclusions

Any significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
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