Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2014/12/15: Difference between revisions

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==Protocol==
==Protocol==
[http://openwetware.org/wiki/Haynes:ThawingCells example protocol]<br><br>
[http://openwetware.org/wiki/Haynes:AdvancingCells advancing adherent cells]
[http://openwetware.org/wiki/Haynes:SplittingCells passaging adherent cells]<br><br>


==Materials==
==Materials==

Revision as of 09:09, 15 December 2014

RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

Explain what you're doing today and why. Paragraph format, simple description so somebody reading the notebook has some orientation.

Goals

  • Passage U2OS cells from PS-β to PS-1
  • Advance SKNSH cells from PS-α to PS-β

Protocol

advancing adherent cells passaging adherent cells

Materials

  • List materials here, to be gathered before the experiment
  • Consumables such as media, antibiotics, DNA, kits etc.

Notes

Observations, measurements, placeholder info etc. go here

Results/Conclusions

Any significant data goes here. Thoughts on what happened, significance of findings, what to do next etc.
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