Haynes Lab:Notebook/Quorum Sensing/2014/09/12: Difference between revisions
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==Entry title== | ==Entry title== | ||
* | * (plasmids not for quorum sensing project) | ||
miniprepped liquid cultures (two samples for each plasmid) | |||
Double-Digested ~750ng each plasmid using EcoRI and PstI in NEB buffer 3 for 30 minutes at 37C. | |||
Ran digestions on a gel. | |||
[[Image:annotated fc4 and kah121 digest e+p.png]] | |||
Revision as of 11:07, 14 September 2014
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Entry title
miniprepped liquid cultures (two samples for each plasmid) Double-Digested ~750ng each plasmid using EcoRI and PstI in NEB buffer 3 for 30 minutes at 37C.
Ran digestions on a gel.
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