Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/09/21: Difference between revisions

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| Plasmid || OD260 || OD260/280 || ng/μL
| Plasmid || OD260 || OD260/280 || ng/μL
|-
|-
| 1. Plasmid 1 || .027 || 1.971 || 26.921
| 1. AmCyan || .027 || 1.971 || 26.921
|-
|-
| 2. Plasmid 2 || .03 || 1.895 || 29.764
| 2. flyPCD || .03 || 1.895 || 29.764
|}
|}


Revision as of 14:48, 21 September 2013

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09/21/13

  • Growth on streak plate
  • AmCyan and flyPCD
    • Inoculated: 9/20/13
    • Culture 1(AmCyan): Growth
    • Culture 2(flyPCD): Growth

Samples For Miniprep and Analysis

Sample 1: Colony 1, BBa_J176022, part = 687, vector = 4968 Sample 2: Colony 2, BBa_J176001, part = 186, vector = 4968

List of Samples

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. AmCyan .027 1.971 26.921
2. flyPCD .03 1.895 29.764


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
 Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.


DNA Sequencing Samples

  • Submitted to DNASU on: 'date'
  • Order Number:
  1. Plasmid 1 - forward primer 'name'
  2. Plasmid 1 - reverse primer 'name'
  3. Plasmid 2 - forward primer 'name'
  4. Plasmid 2 - reverse primer 'name'




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