Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/06/25: Difference between revisions
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(Autocreate 2013/06/25 Entry for Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi) |
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== | ==DH5α Transformation with KAH013 Plasmid 6/25/2013== | ||
#In ice bath, 1μl KAH013 plasmid and 50μl competent E.Coli were combined. | |||
#Waited 10 minutes for bacteria to accept plasmid. | |||
#Transformed bacteria were then added to Agarose gel petri dish, rolling beads were used to evenly spread bacteria. | |||
#Petri dish was labeled with initials date and plasmid name("PK 6/25/2013 KAH13") and was placed in incubator at 37 degrees for two days(6/25-6/26). | |||
#Resultant colonies were picked by Rene and grown in liquid LB media overnight(6/27/2013). | |||
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Revision as of 20:51, 30 June 2013
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DH5α Transformation with KAH013 Plasmid 6/25/2013
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