Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi

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(Autocreated Lab Notebook name=Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi, content from MediaWiki:ProjectContentDefault)
Current revision (18:13, 4 August 2013) (view source)
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==Project Description/Abstract==
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==Project Description==
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* Place short description of project or notes regarding this project
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This notebook consists of the procedures learnt during lab training. DH5α will be transformed with KAH013 plasmid, and then miniprep will be performed, and a spectrophotometer will be used to analyze the DNA yield, and if the process was successful. In order to validate the plasmid, restriction digest and electrophoresis will be employed.
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* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
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'''
==Notes==
==Notes==
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* Place some notes here for visitors
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* Transformation of DH5α was successful. Colonies were growing in agar+amp gel.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/25 6/25/13]
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* Example: This project is currently on hold until further notice.
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*Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/06/28#Spectrophotometer_analysis 6/28/13]
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*Plasmid was successfully validated using restriction digest and electrophoresis.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/02 7/02/13]
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'''Second Transformation Attempt'''
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*DH5α was transformed, but was incorrectly grown in LB-amp plates instead of LB+amp plates.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/24 7/24/13]
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*DH5α was transformed, and was correctly grown in LB+amp plates.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/27 7/26/13]
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*Colony was selected from the plate, and transferred to liquid media.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi/2013/07/27 7/27/13]

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Project Description

This notebook consists of the procedures learnt during lab training. DH5α will be transformed with KAH013 plasmid, and then miniprep will be performed, and a spectrophotometer will be used to analyze the DNA yield, and if the process was successful. In order to validate the plasmid, restriction digest and electrophoresis will be employed.

Notes

  • Transformation of DH5α was successful. Colonies were growing in agar+amp gel.

6/25/13

  • Yield from miniprep was low at 5.542ng/μL. Expected yield was from 20-80ng/μL. Yield reading may have been due to contamination.

6/28/13

  • Plasmid was successfully validated using restriction digest and electrophoresis.

7/02/13

Second Transformation Attempt

  • DH5α was transformed, but was incorrectly grown in LB-amp plates instead of LB+amp plates.

7/24/13

  • DH5α was transformed, and was correctly grown in LB+amp plates.

7/26/13

  • Colony was selected from the plate, and transferred to liquid media.

7/27/13


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