Haynes Lab:Notebook/Jan/2015/04/20: Difference between revisions

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==Passaging Cell Lines ==
==Passaging U2OS Cells to Prepare for Creating a Stable Cell Line==
* Cell Line: U2OS
* Cell Line: U2OS
* BD004 Benchling link:
* BD004 Benchling link:
* BD006 Benchling link:
* BD006 Benchling link:
* Protocol: Passage cells at a 1:10, 1:20, 1:40 dilution into fresh antibiotic-free growth medium 48 hours after transfection.
* Protocol: Passage cells at a 1:10, 1:20, 1:40 dilution into fresh antibiotic-free growth medium 48 hours after transfection. We are passaging at 1:10, 1:20, and 1:40 of each plasmid into 10 cm plates.




Latest revision as of 00:55, 27 September 2017

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Passaging U2OS Cells to Prepare for Creating a Stable Cell Line

  • Cell Line: U2OS
  • BD004 Benchling link:
  • BD006 Benchling link:
  • Protocol: Passage cells at a 1:10, 1:20, 1:40 dilution into fresh antibiotic-free growth medium 48 hours after transfection. We are passaging at 1:10, 1:20, and 1:40 of each plasmid into 10 cm plates.



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