Haynes Lab:Notebook/Jan/2015/04/01: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Jan Simper (talk | contribs) |
Jan Simper (talk | contribs) |
||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Fluorescence Microscopy of Transfected U2OS Cells w/ BD006== | ==Fluorescence Microscopy of Transfected U2OS Cells w/ BD006== | ||
* Cell Line: U2OS | |||
* Plasmid name: BD006 in MV2 | |||
* Benchling Link for Plasmid: [https://benchling.com/hayneslab/f/5Clz1JBiCH-mammalian-constructs/seq-W1AJJvtk-chromatinsensor1_mv2/edit BD006/Chromatin Sensor 1] | |||
* Name of Kit Used for Transfection: Lipofectamine LTX | |||
* FACS needs to be performed to get exact transfection efficiency, but based on pictures, suspected efficiency is 20-30%. Cell morphology does not look great, probably due to the fact that there were few cells on the 6-well plate at the start of transfection. | |||
*[[Image:U2OS transfected BD006 Well 1 phase CFP 10x Image 2 2015-04-01.jpg|600x600px|]] | *[[Image:U2OS transfected BD006 Well 1 phase CFP 10x Image 2 2015-04-01.jpg|600x600px|]] | ||
Revision as of 16:57, 4 April 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Fluorescence Microscopy of Transfected U2OS Cells w/ BD006
|