Haynes Lab:Notebook/Jan/2015/04/01: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==Fluorescence Microscopy of Transfected U2OS Cells w/ BD006==
==Fluorescence Microscopy of Transfected U2OS Cells w/ BD006==
*[[Image:U2OS transfected BD006 Well 1 phase CFP 10x Image 2 2015-04-01.jpg]]
* Cell Line: U2OS
* Plasmid name: BD006 in MV2
* Benchling Link for Plasmid: [https://benchling.com/hayneslab/f/5Clz1JBiCH-mammalian-constructs/seq-W1AJJvtk-chromatinsensor1_mv2/edit BD006/Chromatin Sensor 1]
* Name of Kit Used for Transfection: Lipofectamine LTX
* FACS needs to be performed to get exact transfection efficiency, but based on pictures, suspected efficiency is 20-30%. Cell morphology does not look great, probably due to the fact that there were few cells on the 6-well plate at the start of transfection.


*[[Image:U2OS transfected BD006 Well 1 phase CFP 10x Image 2 2015-04-01.jpg|600x600px|]]


*[[Image:U2OS transfected BD006 Well 2 phase CFP 10x 2015-04-01.jpg]]


*[[Image:U2OS transfected BD006 Well 2 phase CFP 10x 2015-04-01.jpg|600x600px|]]


*[[Image:U2OS transfected BD006 Well 3 phase CFP 10x 2015-04-01.jpg]]
 
*[[Image:U2OS transfected BD006 Well 3 phase CFP 10x 2015-04-01.jpg|600x600px|]]


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Latest revision as of 00:52, 27 September 2017

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Fluorescence Microscopy of Transfected U2OS Cells w/ BD006

  • Cell Line: U2OS
  • Plasmid name: BD006 in MV2
  • Benchling Link for Plasmid: BD006/Chromatin Sensor 1
  • Name of Kit Used for Transfection: Lipofectamine LTX
  • FACS needs to be performed to get exact transfection efficiency, but based on pictures, suspected efficiency is 20-30%. Cell morphology does not look great, probably due to the fact that there were few cells on the 6-well plate at the start of transfection.




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