Haynes Lab:Notebook/Jan/2015/03/28: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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** Elute & back-elute w/ 70 μL elution sln. | ** Elute & back-elute w/ 70 μL elution sln. | ||
# BD006/MV2 plate 1, a | # BD006/MV2 plate 1, a | ||
# BD006/MV2 plate 1, b | |||
# BD006/MV2 plate 2, a | |||
# BD006/MV2 plate 2, b | |||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | Expected:<br>* BD006/MV2 = | | rowspan="7" | Expected:<br>* BD006/MV2 = 7207, 1243, 288<br>* MV2 only = 2998, 1243, 288<br>* BD006/V0120 = 7036, 497 | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:KAH032815_gel1.jpg|270px|Hover name]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| DNA (plasmid) || | | DNA (plasmid) || 3.0 μL | ||
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| 10x buffer || 1.5 | | 10x buffer || 1.5 | ||
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| ApaLI || 1.0 | | ApaLI || 1.0 | ||
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| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 9.5 | ||
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| || 15 μL --> 37°C/ ~10 min. | | || 15 μL --> 37°C/ ~10 min. | ||
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* Conclusions | * Conclusions | ||
** | ** Success! All four look like BD006/MV2 | ||
** Submit for sequencing next week | |||
* Miniprep conc.'s | |||
{| {{table}} cellspacing="3" <!-- [DNA] table --> | |||
|- bgcolor=#cfcfcf | |||
| Sample || OD260 || 260/280 || ng/μL | |||
|- | |||
| 1. BD006/MV2 1-a || 0.274 || 1.44 || 274.10 | |||
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| 2. BD006/MV2 1-b || 0.299 || 1.93 || 298.68 | |||
|- | |||
| 3. BD006/MV2 2-a || 0.331 || 1.93 || 330.69 | |||
|- | |||
| 4. BD006/MV2 2-b || 0.306 || 1.90 || 305.94 | |||
|} | |||
Latest revision as of 00:52, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||
03/28/15
Minipreps - BD006/MV2 colonies
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