Haynes Lab:Notebook/Jan/2015/03/27: Difference between revisions

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* Conclusions
* Conclusions
** MV2 is correct
** tba
** BD004 - most likely missing the D11.12 fragment. Sequence results from Meli'sa's past work agrees with the short fragment size on the gel.
** BD006  is correct


* Miniprep conc.'s
* Miniprep conc.'s
Line 54: Line 52:
| Sample || OD260 || 260/280 || ng/μL
| Sample || OD260 || 260/280 || ng/μL
|-
|-
| 1. MV2 mp || 0.132 || 1.85 || 132.262
| 1. DPRE a mp || 0.411 || 1.89 || 411.47
|-
|-
| 2. BD004 mp || 0.144 || 1.91 || 143.967
| 2. DPRE b mp || 0.411 || 1.89 || 410.51
|-
| 3. BD006 mp || 0.078 || 1.83 || 78.413
|}
|}


Revision as of 16:21, 27 March 2015

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03/27/15

  • Cultures - BD006/MV2 colonies (from 3/26/15 ligations), 5 mL each (4 total)
  • Plasmids - DPRE minipreps (5 mL cultures)

Plasmids - DPRE minipreps

  • ---Karmella 15:12, 27 March 2015 (EDT):
  • Sigma GenElute Miniprep kit.
  • Elute with 70μL elution buffer
  1. DPRE a
  2. DPRE b
  • Digests (Fermentas FD)
    • E/S to prep for insertion upstream of BD004
    • Also digest BD004 w/ E/X
Reagent Volume Expected:
1,2. DPRE (E/S) = ###, 3200
3. BD004 (E/X) = 7318 		Hover name
30 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20.0 μL
10x buffer 3.0
enzyme 1 1.0
enzyme 2 1.0
dH2O ---
  30 μL --> 37°C/ ~30 min.


  • Conclusions
    • tba
  • Miniprep conc.'s
    • Combined the pairs of preps. Pairs look identical.
Sample OD260 260/280 ng/μL
1. DPRE a mp 0.411 1.89 411.47
2. DPRE b mp 0.411 1.89 410.51



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