Haynes Lab:Notebook/Jan/2015/03/20: Difference between revisions
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** BD004 - most likely missing the D11.12 fragment. Sequence results from Meli'sa's past work agrees with the short fragment size on the gel. | ** BD004 - most likely missing the D11.12 fragment. Sequence results from Meli'sa's past work agrees with the short fragment size on the gel. | ||
** BD006 is correct | ** BD006 is correct | ||
* Miniprep conc.'s | |||
** Combined the pairs of preps. Pairs look identical. | |||
{| {{table}} cellspacing="3" <!-- [DNA] table --> | |||
|- bgcolor=#cfcfcf | |||
| Sample || OD260 || 260/280 || ng/μL | |||
|- | |||
| 1. MV2 mp || 0.132 || 1.85 || 132.262 | |||
|- | |||
| 2. BD004 mp || 0.144 || 1.91 || 143.967 | |||
|- | |||
| 3. BD006 mp || 0.078 || 1.83 || 78.413 | |||
|} | |||
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| Sample || OD260 || 260/280 || ng/μL | | Sample || OD260 || 260/280 || ng/μL | ||
|- | |- | ||
| 1. MV2 (E/X) || | | 1. MV2-band1 (E/X) || 0.033 || 0.58 || 32.94 | ||
|- | |- | ||
| 2. MV2 (E/X) || - | | 2. MV2-band2 (E/X) || -0.01(?) || --- || --- | ||
|- | |- | ||
| 3. BD006 (E/S) || - | | 3. BD006-band1 (E/S) || -0.005(?) || --- || --- | ||
|- | |- | ||
| 4. BD006 (E/S) || - | | 4. BD006-band2 (E/S) || -0.01(?) || --- || --- | ||
|} | |} | ||
Revision as of 17:19, 26 March 2015
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03/20/15
Plasmids - minipreps & digests
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