Haynes Lab:Notebook/Jan/2015/03/18: Difference between revisions
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==Plasmid Transformations of MV2, BD004, BD006== | ==Plasmid Transformations of MV2, BD004, BD006== | ||
*MV2 | |||
*Plasmid name: MV2 | |||
*BioBrick name: None | |||
*Benchling link: [https://benchling.com/hayneslab/f/EQNhAGU4wP-vectors-for-mammalian-expression/seq-DpuwS4to-mv2/edit MV2] | |||
*Ampicillin resistance | |||
*Length of plasmid: 4529 bp | |||
*Transformation done on 3/17/2015 | *Transformation done on 3/17/2015 | ||
*Result for Plate 1: Good growth over all of the plate. Overall success. | *Result for Plate 1: Good growth over all of the plate, some overgrowth. Overall success. | ||
*Result for Plate 2: No growth anywhere. Success. | *Result for Plate 2: No growth anywhere. Success. | ||
*Plasmid name: BD004 | |||
*BD004 | *BioBrick name: None (composed of DPRE-5xGal4-spacer-HPK-Kozak-AmCyan-AmCyan-NLS-Stop-PolyA) | ||
*Benchling link: [https://benchling.com/hayneslab/f/5Clz1JBiCH-mammalian-constructs/seq-AZA653Ud-chromatin-sensor-2/ BD004] | |||
*Ampicillin resistance | |||
*Length of plasmid: 4116 bp | |||
*Transformation done on 3/17/2015 | *Transformation done on 3/17/2015 | ||
*Result for Plate 1: Good growth over all of the plate. Overall success. | *Result for Plate 1: Good growth over all of the plate, some overgrowth. Overall success. | ||
*Result for Plate 2: No growth anywhere. Success. | *Result for Plate 2: No growth anywhere. Success. | ||
*BD006 | *Plasmid name: BD006 | ||
*BIobrick name: None (composed of DPRE-5xGal4-spacer-CMV-Kozak-AmCyan-AmCyan-NLS-Stop-PolyA) | |||
*Benchling link: [https://benchling.com/hayneslab/f/5Clz1JBiCH-mammalian-constructs/seq-ohc5j4Jm-chromatin-sensor-1/ BD006] | |||
*Ampicillin resistance | |||
*Length of plasmid: 4182 bp | |||
*Transformation done on 3/17/2015 | *Transformation done on 3/17/2015 | ||
*Result for Plate 1: Good growth over all of the plate. Overall success. | *Result for Plate 1: Good growth over all of the plate, some overgrowth. Overall success. | ||
*Result for Plate 2: No growth anywhere. Success. | *Result for Plate 2: No growth anywhere. Success. | ||
Revision as of 12:37, 19 March 2015
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Plasmid Transformations of MV2, BD004, BD006
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