Extraction and Verification of mCh
- Name: mCh / BBa_J176005
- Date of culture inoculation: 10/17/13
- Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
- Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although more growth in colony 1 streak than colony 2 streak. Overall success.
- Sample 1: mCh/V0120 colony 1; BBa_J176005; part = 705 bp; vector = 3200 bp
- Sample 2: mCh/V0120 colony 2; same
- Sample 3: mCh/V0120 (control DNA); BBa_J176005; part = 705 bp; vector = 3200 bp
DNA Concentration Data
Plasmid |
OD260 |
OD260/280 |
ng/μL
|
1. Plasmid 1 |
0.12 |
1.84 |
120
|
2. Plasmid 2 |
0.026 |
1.656 |
26
|
Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
Reagent
|
Volume
|
Expected: 1. Plasmid 1 = 705, 3200 2. Plasmid 2 = 705 bp, 3200 bp
|
15 μL/lane; 1% agarose; Ladder
|
DNA(plasmid) |
3.0 μL
|
10X buffer |
1.5
|
EcoRI |
1.0
|
PstI |
1.0
|
dH2O |
8.5
|
|
15 μL --> 37°C/ 15 min.
|
DNA Sequencing Samples
- Submitted to DNASU on: 'date'
- Order Number:
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
|