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Extraction and Verification of mCh
- Name: mCh / BBa_J176005
- Date of culture inoculation: 10/17/13
- Results for Cultures 1 and 2: Mixture is cloudy so growth in both. Success.
- Results for Streaks 1 and 2: Growth in both areas along zig-zag line, although more growth in colony 1 streak than colony 2 streak. Overall success.
- Sample 1: mCh/V0120 colony 1; BBa_J176005; part = 705 bp; vector = 3200 bp
- Sample 2: mCh/V0120 colony 2; same
- Sample 3: mCh/V0120 (control DNA); BBa_J176005; part = 705 bp; vector = 3200 bp
DNA Concentration Data
| Plasmid |
OD260 |
OD260/280 |
ng/μL
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| 1. Plasmid 1 |
--- |
--- |
---
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| 2. Plasmid 2 |
--- |
--- |
---
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Restriction Digest Table
- Checked plasmid minipreps with EcoRI/PstI digests
| Reagent
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Volume
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Expected:
1. Plasmid 1 = insert size, vector size
2. Plasmid 2 = insert size, vector size
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15 μL/lane; 1% agarose; Ladder
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| DNA(plasmid) |
3.0 μL
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| 10X buffer |
1.5
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| EcoRI |
1.0
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| PstI |
1.0
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| dH2O |
8.5
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15 μL --> 37°C/ 15 min.
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DNA Sequencing Samples
- Submitted to DNASU on: 'date'
- Order Number:
- Plasmid 1 - forward primer 'name'
- Plasmid 1 - reverse primer 'name'
- Plasmid 2 - forward primer 'name'
- Plasmid 2 - reverse primer 'name'
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