Haynes Lab:Notebook/Jan/2013/10/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Growing Liquid Culture of mCh== | ||
* | * mCh / BBa_J176005 | ||
** Transformation done on [http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan/2013/10/15 10/15/13] | |||
** Result for Plate 1: Good growth over much of the plate. Overall success. | |||
** Result for Plate 2: Growth over about half of the plate, indicating an error in procedure. | |||
Because there was growth on the negative control plate, indicating a fault in the transformation, the plates were disposed of and the transformation was redone. | |||
* Plasmid name: mCh in V0120 vector | |||
** BioBrick name: BBa_J176005 [http://parts.igem.org/Part:BBa_J176005 BBa_J176005] | |||
** Vector name: V0120 (BBa_J176127) | |||
** Ampicillin resistance on V0120 | |||
** Length of vector: 3200 bp | |||
* Sample 1: Plasmid DNA - mCh (BBa_J176005) | |||
* Sample 2: negative control - water (Vol 1, p. 166) | |||
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Latest revision as of 23:29, 26 September 2017
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Growing Liquid Culture of mCh
Because there was growth on the negative control plate, indicating a fault in the transformation, the plates were disposed of and the transformation was redone.
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