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Restriction Digest and Gel Electrophoresis
Plasmid DNA 25.0 μl* Fermentas FastDigest enzyme 1 1.0 μl Fermentas FastDigest enzyme 2 1.0 μl 10x FastDigest buffer + green loading dye 3.0 μl dH2O 0.0 μl
30.0 μl total
The maximum amount of plasmid DNA was used and no dH2O was used because of the low concentration of the plasmid DNA (22 ng/μl).