Haynes Lab:Notebook/Jan/2013/06/28: Difference between revisions
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* 3. At this stage, protocol continued because the tubes were not marked when centrifuged in step 2; however, if we were certain about which tubes were which, the remaining fluid from the incubation tube should be poured into the 2 mL centrifuge tube and steps 1-2 repeated to get as much DNA as possible into the 2 mL centrifuge tube. The lack of this step may have contributed to the later results (see below). | * 3. At this stage, protocol continued because the tubes were not marked when centrifuged in step 2; however, if we were certain about which tubes were which, the remaining fluid from the incubation tube should be poured into the 2 mL centrifuge tube and steps 1-2 repeated to get as much DNA as possible into the 2 mL centrifuge tube. The lack of this step may have contributed to the later results (see below). | ||
* 4. At this stage, the Zyppy Plasmid Protocol was followed as in this image: [[Image:Lab01.jpg]] | * 4. At this stage, the Zyppy Plasmid Protocol was followed as in this image: [[Image:Lab01.jpg|200px|]] However, the protocol is repeated in writing here. | ||
* 5. Add 100 μL 7x lysis buffer (blue) to the centrifuge tube with 600 ul of fluid and invert 4-6 times, allowing 1-2 minutes for lysis. Time limit 2 minutes for lysis. | * 5. Add 100 μL 7x lysis buffer (blue) to the centrifuge tube with 600 ul of fluid and invert 4-6 times, allowing 1-2 minutes for lysis. Time limit 2 minutes for lysis. |
Revision as of 15:51, 5 July 2013
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June 28th, 2013
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