Haynes Lab:Notebook/Jan

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==Project Description==
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==Using Public Data to Predict and Control Stem Cell Development==
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* This is the lab notebook of me, Jan Simper, an undergraduate currently working in the Haynes Lab at Arizona State University. Any and all activities I do in the lab will be recorded here.
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* Currently, the changes in epigenetic state that occur when a differentiated adult cell is converted into an induced pluripotent stem cell (iPS cell) are not well understood. Distinct tissue or cell types have various bioengineering requirements to ensure full reprogramming towards the pluripotent ground state and then back into a specialized type of tissue or cell. Thus there exists a need to refine tissue-specific differentiation protocols by identifying the appropriate somatic tissue source and reprogramming strategy.
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* This project will serve to identify genes that are necessary for stem cell differentiation through bioinformatics and to confirm the role of these genes in a laboratory setting.
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* This FURI project has been approved for Fall 2014 and Spring 2015.
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[http://openwetware.org/wiki/Haynes_Lab:Notebook/Jan/2013/08/12 8/12/2013] Restriction Digest and Gel Electrophoresis
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Using Public Data to Predict and Control Stem Cell Development

  • Currently, the changes in epigenetic state that occur when a differentiated adult cell is converted into an induced pluripotent stem cell (iPS cell) are not well understood. Distinct tissue or cell types have various bioengineering requirements to ensure full reprogramming towards the pluripotent ground state and then back into a specialized type of tissue or cell. Thus there exists a need to refine tissue-specific differentiation protocols by identifying the appropriate somatic tissue source and reprogramming strategy.
  • This project will serve to identify genes that are necessary for stem cell differentiation through bioinformatics and to confirm the role of these genes in a laboratory setting.
  • This FURI project has been approved for Fall 2014 and Spring 2015.
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