Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/05/02

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Current revision (10:10, 7 May 2013) (view source)
(May 2, 2013)
 
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* Transformation of the site-directed mutagenesis was retried with 1 μL of the product and 30 μL of DH5α
* Transformation of the site-directed mutagenesis was retried with 1 μL of the product and 30 μL of DH5α
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[[Image:VN_Mutagenesis_Transformation_Trial_2_DH5a.png‎|thumb|frame|center|The next day, no colonies were seen for the H2B mutagenesis but there were about 25 colonies for the LOV mutagenesis.]]
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[[Image:VN_Mutagenesis_Transformation_Trial_2_DH5a.png‎|thumb|frame|center|The next day, no colonies were seen for the H2B mutagenesis but there were about 20 colonies for the LOV mutagenesis.]]

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May 2, 2013

  • Transformation of the site-directed mutagenesis was retried with 1 μL of the product and 30 μL of DH5α
The next day, no colonies were seen for the H2B mutagenesis but there were about 20 colonies for the LOV mutagenesis.
The next day, no colonies were seen for the H2B mutagenesis but there were about 20 colonies for the LOV mutagenesis.



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