Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/04/22
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 19, 2013Quikchange Site Directed Mutagenesis
which includes 5 control reactions.
to multiple freeze-thaw cycles.
100 mM KCl || 100 mM(NH4)2SO4 || 200 mM Tris-HCl (pH 8.8) || 20 mM MgSO4 || 1% Triton® X-100 || 1 mg/ml nuclease-free bovine serum albumin (BSA) ||
10 mM Tris-HCl (pH 7.5) 1 mM EDTA
5 μl of 10x reaction buffer 2 μl (10 ng) of pWhitescript 4.5-kb control template (5 ng/μl) 1.25 μl (125 ng) of oligonucleotide control primer #1 [34-mer (100 ng/μl)] 1.25 μl (125 ng) of oligonucleotide control primer #2 [34-mer (100 ng/μl)] 1 μl of dNTP mix ddH2O to a final volume of 50 μl
5 μl of 10× reaction buffer X μl (5–50 ng) of dsDNA template X μl (125 ng) of oligonucleotide primer #1 X μl (125 ng) of oligonucleotide primer #2 1 μl of dNTP mix ddH2O to a final volume of 50 μl
|