Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/02/11

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February 9, 2013

Golden Gate Assembly

  • Mediated assembly
    • The volume of purified DNA (x) needed to dilute in a final volume of 20 μL = length in bp ÷ measured ng/μL * 20 fmols/μL * 650 fg/fmol ÷ 1,000,000 fg/ng * 20 μL final volume
    • Formula: x = length in bp ÷ measured ng/μL * 0.013 * 20
DNA length (bp) ng/μL vol. dH2O
H2B 410 77.995 1.4 18.6
LOV 461 80.443 1.5 18.5
pSB1A3 2000 77.4 6.7 13.3



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