Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/02/08: Difference between revisions
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* Final assembly product: pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | * Final assembly product: pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | ||
* Primer design | * Primer design | ||
H2B | #H2B | ||
1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG | 1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG | ||
H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA | H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA | ||
LOV | #LOV | ||
LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT | LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT | ||
LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC | LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC | ||
Revision as of 05:40, 8 February 2013
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February 8, 2013Type IIS Assembly - Making PCR Products
1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA
LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC
PCR program:
Results of the PCR product digests are shown, with H2B on the left and LOV on the right. The gel was loaded with 5 μL samples.
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