Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/02/08
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==February 8, 2013== | ==February 8, 2013== | ||
| - | '''Type IIS Assembly | + | '''Type IIS Assembly - Making PCR Products ''' |
* Attempt new assembly method because piecewise assembly was unsuccessful | * Attempt new assembly method because piecewise assembly was unsuccessful | ||
* Final assembly product: pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | * Final assembly product: pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | ||
Revision as of 08:39, 8 February 2013
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February 8, 2013Type IIS Assembly - Making PCR Products
H2B 1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA LOV LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC
PCR program:
Results of the PCR product digests are shown, with H2B on the left and LOV on the right. The gel was loaded with 5 μL samples.
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