Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2013/02/08: Difference between revisions
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[[Image: | [[Image:VN_Gel_2-8-13.png|Results of the digests are shown. It was later found that the transparency used was blocking the UV light.]] | ||
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | [[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | ||
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| PCR Product || 260|| 280 || 260/280 || ng/μL | | PCR Product || 260|| 280 || 260/280 || ng/μL | ||
|- | |- | ||
| H2B || | | H2B || 0.078|| 0.043|| 1.81 || 77.995 | ||
|- | |||
| LOV || 0.08 || 0.044 || 1.817 || 80.443 | |||
|- | |||
| pSB1A3 || 0.023 || 0.014 || 1.727 || 23.386 | |||
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|} | |} | ||
Revision as of 05:51, 18 April 2013
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February 8, 2013Type IIS Assembly - Making PCR Products
1A3/H2B fwd 5'-cacaccaCGTCTCa TAGA ATGCCAGAGCCAGCG H2B/LOV rev 5'-cacaccaCGTCTCa CCAA CTTAGCGCTGGTGTA
LOV fwd 5'-cacaccaCGTCTCa TTGGCTACTACACTT LOV+his/1A3 rev 5'-cacaccaCGTCTCa TAGT ttagtggtgatggtgatgatgAAGTTCTTTTGCCGC
PCR program:
Results of the PCR product digests are shown, with H2B on the left and LOV on the right. The gel was loaded with 5 μL samples.
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