Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/07: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | ||
[[Image:VN_Gibson_Assembly_Trial_2.png|thumb|frame|center|There were some pseudo colonies seen the next day but these had a white film so they probably did not contain the ligation.]] | |||
Latest revision as of 22:19, 26 September 2017
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December 7, 2012
Digestion
Results of the enzyme digest is shown, with both pSB1A3 bands on the right. The gel was loaded with 30 μL samples. The bands that were cut are outlined in green.
Ligation
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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