Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/06: Difference between revisions
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Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | ||
[[Image: | [[Image:VN_Gibson_Assembly_Trial_1.png|thumb|frame|center|No colonies were seen the next day.]] | ||
'''Made more pSB1A3 stock''' | |||
{| class="wikitable" border=1 cellpadding="5" cellspacing="0" | |||
|- | |||
|Trial || 260 || 280 || 260/280 || ng/μL | |||
|- | |||
|1 || 0.062|| 0.035 || 1.773|| 62.46 | |||
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|2 || 0.052|| 0.03 || 1.722|| 52.032 | |||
|- | |||
|3 || 0.082|| 0.047|| 1.762|| 82.222 | |||
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|} | |||
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Revision as of 05:37, 18 April 2013
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December 6, 2012Assemblies
Dephosphorylation (11/23/12) > Dephosphorylation (Roche)
Ligation
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 Made more pSB1A3 stock
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