Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/06: Difference between revisions
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| Gibson master mix || 15.0 || 15.0 || 15.0 | | Gibson master mix || 15.0 || 15.0 || 15.0 | ||
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| Total volume || 20.0 || 20.0 || 18.0 | |||
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| colspan="3" | Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 50°C for 1 hour. | | colspan="3" | Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 50°C for 1 hour. | ||
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Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 | ||
[[Image:VN_Gibson_Assembly_Trial_1.png|thumb|frame|center|No colonies were seen the next day.]] | |||
'''Made more pSB1A3 stock''' | |||
{| class="wikitable" border=1 cellpadding="5" cellspacing="0" | |||
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|Trial || 260 || 280 || 260/280 || ng/μL | |||
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|1 || 0.062|| 0.035 || 1.773|| 62.46 | |||
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|2 || 0.052|| 0.03 || 1.722|| 52.032 | |||
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|3 || 0.082|| 0.047|| 1.762|| 82.222 | |||
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Revision as of 05:37, 18 April 2013
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December 6, 2012Assemblies
Dephosphorylation (11/23/12) > Dephosphorylation (Roche)
Ligation
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101 Made more pSB1A3 stock
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