Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/06: Difference between revisions
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| Gibson master mix || 15.0 || 15.0 || 15.0 | | Gibson master mix || 15.0 || 15.0 || 15.0 | ||
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| Total volume || 20.0 || 20.0 || 18.0 | |||
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| colspan="3" | Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 50°C for 1 hour. | | colspan="3" | Mix the reaction(s) thoroughly by flicking the tube.<br>Incubate at 50°C for 1 hour. |
Revision as of 07:00, 8 December 2012
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December 6, 2012Assemblies
Dephosphorylation (11/23/12) > Dephosphorylation (Roche)
Ligation
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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