Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/12/05: Difference between revisions
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* Note: Primers add a XbaI site upstream and the SpeI site downstream | * Note: Primers add a XbaI site upstream and the SpeI site downstream | ||
* Final Gibson assembly products | * Final Gibson assembly products | ||
#pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | # pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end | ||
#pSB1A3 left end / LOV / H2B +His tag/ pSB1A3 right end | # pSB1A3 left end / LOV / H2B +His tag/ pSB1A3 right end | ||
*For H2B-LOV construct | *For H2B-LOV construct |
Revision as of 16:51, 5 December 2012
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December 5, 201211/21/12Results from last week's ligation
# pSB1A3 left end / H2B / LOV +His tag/ pSB1A3 right end # pSB1A3 left end / LOV / H2B +His tag/ pSB1A3 right end
Performed table reactions twice: once for those used in H2B-LOV construct, once for those used in LOV-H2B construct
PCR program:
Assemblies
> Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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