Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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{| class="wikitable" width= | {| class="wikitable" width=700px | ||
| || H2B || LOV || Negative Control | | || H2B || LOV || Negative Control || H2B || LOV || Negative Control | ||
|- | |- | ||
| Insert DNA (2x mol vector) || 1.0 μL || 1.0 || --- || 1.0 || 1.0 || --- | | Insert DNA (2x mol vector) || 1.0 μL || 1.0 || --- || 1.0 || 1.0 || --- |
Revision as of 14:50, 23 November 2012
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11/21/12Results from yesterday's ligation
PCR program:
Assemblies
> Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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