Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(3 intermediate revisions by the same user not shown) | |||
Line 90: | Line 90: | ||
| Sample|| 260|| 260/280 || ng/μL | | Sample|| 260|| 260/280 || ng/μL | ||
|- | |- | ||
| H2B - X/S || | | H2B - X/S 1 || 0.016 ||1.807|| 15.961 | ||
|- | |- | ||
| LOV - X/S | | H2B - X/S 2 || 0.009 || 1.976 || 8.619 | ||
|- | |||
| LOV - X/S 1 || 0.006|| 1.694 || 6.455 | |||
|- | |||
| LOV - X/S 2 || 0.025|| 1.883 || 25.363 | |||
|} | |} | ||
[[Image:VN_Gel_11- | [[Image:VN_Gel_11-21-12.png|Results of from the digests are shown.]] | ||
[[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | [[Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]] | ||
Line 102: | Line 106: | ||
# LOV = 461 | # LOV = 461 | ||
* There were two samples made of each PCR reaction. The two from the H2B are shown on the left, and the two from the LOV are shown on the right. | |||
Revision as of 06:26, 22 November 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/21/12Results from yesterday's ligation
PCR program:
Assemblies
> Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
|