Haynes Lab:Notebook/Investigating Photo-Switchable Synthetic Nucleosomes/2012/11/21: Difference between revisions
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'''Assemblies''' | '''Assemblies''' | ||
# H2B - X/S - 410 bp + pSB1A3 - X/S - 2000 bp | # H2B - X/S - 410 bp + pSB1A3 - X/S - 2000 bp | ||
# LOV - X/S - 461 bp + pSB1A3 - X/S - 2000 bp | |||
# (negtaive control) pSB1A3 - X/S - 2000 bp | |||
* Digests: | * Digests: | ||
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'''Ligation''' | '''Ligation''' | ||
* Use 20 ng of vector for each ligation = '''1.5 μL pSB1A3''' | * Use 20 ng of vector for each ligation = '''1.5 μL pSB1A3''' | ||
* Use 2x moles of H2B insert, relative to vector: xμL H2B insert = 3 * 410 bp H2B / 2000 bp pSB1A3 * 20 ng pSB1A3 / ### ng/μL H2B = ''' | * Use 2x moles of H2B insert, relative to vector: xμL H2B insert = 3 * 410 bp H2B / 2000 bp pSB1A3 * 20 ng pSB1A3 / ### ng/μL H2B = '''### μL H2B''' | ||
* Use 2x moles of LOV insert, relative to vector: xμL LOV insert = 3 * 461 bp LOV / 2000 bp pSB1A3 * 20 ng pSB1A3 / ### ng/μL LOV = ''' | * Use 2x moles of LOV insert, relative to vector: xμL LOV insert = 3 * 461 bp LOV / 2000 bp pSB1A3 * 20 ng pSB1A3 / ### ng/μL LOV = '''### μL LOV''' | ||
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Revision as of 14:23, 21 November 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/21/12
PCR program:
Assemblies
Dephosphorylation > Dephosphorylation (Roche)
Proceed directly to Transformation. See http://openwetware.org/wiki/Haynes:Assembly101
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